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Вопросы вирусологии. 2017; 62: 81-86

Генетическое разнообразие вирусов вида Chenuda virus (Orbivirus, Reoviridae), циркулирующих в Средней Азии

Еремян А. А., Львов Д. К., Щетинин А. М., Дерябин П. Г., Аристова В. А., Гительман А. К., Ботиков А. Г., Альховский С. В.

Аннотация

Chenuda virus (CNUV) (Orbivirus, семейство Reoviridae) является единственным известным видом орбивирусов, экологически связанным с аргасовыми (Argasidae) клещами, и изучение этой группы вирусов необходимо для понимания общих закономерностей эволюции вирусов рода Orbivirus. Проведен сравнительный полногеномный анализ 5 различных вирусов, принадлежащих к виду Chenuda virus, включая штаммы вируса Баку (BAKV), и циркулирующих на относительно ограниченной территории в Центральной Азии и в Эакавказье. Показано, что дивергенция генов белков VP4 (OC1) и VP6 (Hel) значительно превышает этот показатель для остальных генов. Дивергенция по белкам VP4 (OC1) и VP6 (Hel) между CNUV и BAKV составляет около 50%. Даже у близкородственных штаммов, изолированных в одном географическом регионе и обладающих высокой степенью гомологии консервативных генов (90 - 95%), дивергенция по VP4 (OC1) и VP6 (Hel) достигает значений, которые соответствуют разным серотипам (74,1 - 82,2%).
Список литературы

1. Attoui H., Mertens P.P.C., Becnel J., Belaganahalli S., Bergoin M., Brussaard C.P. et al. Family Reoviridae. In: King A.M., Adams M.J., Carstens E.B., Lefkowitz E.J., eds. Virus Taxonomy: Ninth Report of the International Committee of Taxonomy of Viruses. London: Elsevier; 2012: 541-637.

2. Attoui H., Mohd Jaafar F. Zoonotic and emerging orbivirus infections. Rev. Sci. Tech. 2015; 34 (2): 353-61.

3. Львов Д.К. Вирусы и вирусные инфекции человека и животных: руководство по вирусологии. М.: МИА; 2013.

4. Урываев Л.В., Львов Д.К. Реовирусы. В кн.: Львов Д.К., ред. Медицинская вирусология. М.: МИА; 2008: 202-6.

5. Libikova H., Heinz F., Ujhazyova D., Stunzner D. Orbiviruses of the Kemerovo complex and neurological diseases. Med. Microbiol. Immunol. 1978; 166 (1-4): 255-63.

6. Chumakov M.P., Sarmanova E.S., Bychkova M.V., Bannova G.G., Pivanova G.P., Karpovich L.G. et al. Identification of Kemerovo Tick-Borne Fever Virus and Its Antigenic Independence. Fed. Proc. Transl. Suppl. 1964; 23: 852-4.

7. Lvov D.K., Shchelkanov M.Y., Alkhovsky S.V., Deryabin P.G. Zoonotic Viruses of Northern Eurasia: Taxonomy and Ecology. New York, USA: Academic Press; 2014.

8. Karabatsos N., ed. International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates. 3rd ed. San Antonio, Texas: American Society of Tropical Medicine and Hygiene for the Subcommittee on Information Exchange of the American Committee on Arthropod-borne Viruses; 1985.

9. Альховский С.В., Львов Д.К., Щелканов М.Ю., Щетинин А.М., Дерябин П.Г., Гительман А.К. и др. Таксономия вируса Баку (Baku virus, BAKV; Reoviridae, Orbivirus), изолированного из облигатных паразитов птиц - аграсовых клещей (Acari: Argasidae) в Азербайджане, Туркменистане и Узбекистане. Вопросы вирусологии. 2013; 58 (6): 22-6.

10. Андреев В.Л., Громашевский В.Л., Веселовская О.В. Изоляция вируса Баку в Западной части Туркменской ССР. Экология вирусов. 1973; (1): 96-103.

11. Львов Д.К., Громашевский В.Л., Сидорова Г.А., Тучкин Ю.М. Выделение нового арбовируса Баку группы Кемерово из аргасовых клещей Ornithodoros capensis в Азербайджане. Вопросы вирусологии. 1971; (4): 434-7.

12. Сидорова Г.А., Жмаева З.М., Громашевский В.Л. Изоляция вируса Баку от клещей Ornithodoros coniceps, собранных в гнездовьях голубей в Ташкентской области. Экология вирусов. 1974; (2): 102-5.

13. Mertens P.P., Brown F., Sangar D.V. Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology. 1984; 135 (1): 207-17.

14. French T.J., Inumaru S., Roy P. Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells. J. Virol. 1989; 63 (8): 3270-8.

15. Roy P., Marshall J.J., French T.J. Structure of the bluetongue virus genome and its encoded proteins. Curr. Top. Microbiol. Immunol. 1990; 162: 43-87.

16. Belaganahalli M.N., Maan S., Maan N.S., Nomikou K., Pritchard I., Lunt R. et al. Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus. PLoS One. 2012; 7 (3): e31911.

17. Belaganahalli M.N., Maan S., Maan N.S., Nomikou K., Guimera M., Brownlie J. et al. Full genome sequencing of Corriparta virus, identifies California mosquito pool virus as a member of the Corriparta virus species. PLoS One. 2013; 8 (8): e70779.

18. Dilcher M., Hasib L., Lechner M., Wieseke N., Middendorf M., Marz M. et al. Genetic characterization of Tribec virus and Kemerovo virus, two tick-transmitted human-pathogenic Orbiviruses. Virology. 2012; 423 (1): 68-76.

19. Belaganahalli M.N., Maan S., Maan N.S., Tesh R., Attoui H., Mertens P.P. Umatilla virus genome sequencing and phylogenetic analysis: identification of stretch lagoon orbivirus as a new member of the Umatilla virus species. PLoS One. 2011; 6 (8): e23605.

20. Belhouchet M., Mohd Jaafar F., Tesh R., Grimes J., Maan S., Mertens P.P. et al. Complete sequence of Great Island virus and comparison with the T2 and outer-capsid proteins of Kemerovo, Lipovnik and Tribec viruses (genus Orbivirus, family Reoviridae). J. Gen. Virol. 2010; 91 (Pt. 12): 2985-93.

21. Briese T., Calisher C.H., Higgs S. Viruses of the family Bunyaviridae: are all available isolates reassortants? Virology. 2013; 446 (1-2): 207-16.

22. Wenske E.A., Chanock S.J., Krata L., Fields B.N. Genetic reassortment of mammalian reoviruses in mice. J. Virol. 1985; 56 (2): 613-6.

23. Carpi G., Holmes E.C., Kitchen A. The evolutionary dynamics of bluetongue virus. J. Mol. Evol. 2010; 70 (6): 583-92.

24. Coetzee P., Van Vuuren M., Stokstad M., Myrmel M., Venter E.H. Bluetongue virus genetic and phenotypic diversity: towards identifying the molecular determinants that influence virulence and transmission potential. Vet. Microbiol. 2012; 161 (1-2): 1-12.

25. Brown S.E., Morrison H.G., Knudson D.L. Genetic relatedness of the Kemerovo serogroup viruses: III. RNA-RNA blot hybridization and gene reassortment in vitro of the Chenuda serocomplex. Acta Virol. 1989; 33 (3): 221-34.

26. Brown S.E., Morrison H.G., Knudson D.L. Genetic relatedness of the kemerovo serogroup viruses: II. RNA-RNA blot hybridization and gene reassortment in vitro of the Great Island serocomplex. Acta Virol. 1989; 33 (3): 206-20.

27. Belaganahalli M.N., Maan S., Maan N.S., Brownlie J., Tesh R., Attoui H. et al. Genetic characterization of the tick-borne orbiviruses. Viruses. 2015; 7 (5): 2185-209.

28. Belhouchet M., Mohd Jaafar F., Firth A.E., Grimes J.M., Mertens P.P., Attoui H. Detection of a fourth orbivirus non-structural protein. PLoS One. 2011; 6 (10): e25697.

29. Balasuriya U.B., Dobbe J.C., Heidner H.W., Smalley V.L., Navarrette A., Snijder E.J. et al. Characterization of the neutralization determinants of equine arteritis virus using recombinant chimeric viruses and site-specific mutagenesis of an infectious cDNA clone. Virology. 2004; 321 (2): 235-46.

30. White J.R., Boyd V., Brangwyn J.K., Duch C.J., Pritchard L.I., Melville L.F. et al. Characterisation and monitoring of neutralisation-resistant VP2 phenotypes in BTV-1 isolates from northern Australia collected over a twenty-year period. Vet. Ital. 2004; 40 (4): 508-12.

31. Roy P., Adachi A., Urakawa T., Booth T.F., Thomas C.P. Identification of bluetongue virus VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells. J. Virol. 1990; 64 (1): 1-8.

32. Matsuo E., Roy P. Bluetongue virus VP6 acts early in the replication cycle and can form the basis of chimeric virus formation. J. Virol. 2009; 83 (17): 8842-8.

33. Stauber N., Martinez-Costas J., Sutton G., Monastyrskaya K., Roy P. Bluetongue virus VP6 protein binds ATP and exhibits an RNAdependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates. J. Virol. 1997; 71 (10): 7220-6.

Problems of Virology. 2017; 62: 81-86

Genetic diversity of viruses of Chenuda virus species (Orbivirus, Reoviridae) circulating in Central Asia

Eremyan A. A., Lvov D. K., Shchetinin A. M., Deryabin P. G., Aristova V. A., Gitelman A. K., Botikov A. G., Alkhovsky S. V.

Abstract

Chenuda virus (CNUV) (Orbivirus, Reoviridae) is the only known orbivirus associated with argas (Argasidae) ticks. Scientific study of this group is necessary for understanding of Orbivirus genus evolution patterns. We conducted a comparative analysis of full genomes of five different viruses of Chenuda virus species, including Baku virus strains (BAKV) circulating in a rather limited area in the Central Asia and Transcaucasia. It was shown that VP4(OC1) and VP6(Hel) proteins variability greatly exceeds the variability of other proteins. The divergence between CNUV and BAKV in this proteins is about 50%. Even in closely related strains isolated from the same geographical region, the conservative genes of which are 90-95% identical, the VP4(OC1) and VP6(Hel) divergence reaches values that would usually be indicative of different serotypes (74.1-82.2%).
References

1. Attoui H., Mertens P.P.C., Becnel J., Belaganahalli S., Bergoin M., Brussaard C.P. et al. Family Reoviridae. In: King A.M., Adams M.J., Carstens E.B., Lefkowitz E.J., eds. Virus Taxonomy: Ninth Report of the International Committee of Taxonomy of Viruses. London: Elsevier; 2012: 541-637.

2. Attoui H., Mohd Jaafar F. Zoonotic and emerging orbivirus infections. Rev. Sci. Tech. 2015; 34 (2): 353-61.

3. L'vov D.K. Virusy i virusnye infektsii cheloveka i zhivotnykh: rukovodstvo po virusologii. M.: MIA; 2013.

4. Uryvaev L.V., L'vov D.K. Reovirusy. V kn.: L'vov D.K., red. Meditsinskaya virusologiya. M.: MIA; 2008: 202-6.

5. Libikova H., Heinz F., Ujhazyova D., Stunzner D. Orbiviruses of the Kemerovo complex and neurological diseases. Med. Microbiol. Immunol. 1978; 166 (1-4): 255-63.

6. Chumakov M.P., Sarmanova E.S., Bychkova M.V., Bannova G.G., Pivanova G.P., Karpovich L.G. et al. Identification of Kemerovo Tick-Borne Fever Virus and Its Antigenic Independence. Fed. Proc. Transl. Suppl. 1964; 23: 852-4.

7. Lvov D.K., Shchelkanov M.Y., Alkhovsky S.V., Deryabin P.G. Zoonotic Viruses of Northern Eurasia: Taxonomy and Ecology. New York, USA: Academic Press; 2014.

8. Karabatsos N., ed. International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates. 3rd ed. San Antonio, Texas: American Society of Tropical Medicine and Hygiene for the Subcommittee on Information Exchange of the American Committee on Arthropod-borne Viruses; 1985.

9. Al'khovskii S.V., L'vov D.K., Shchelkanov M.Yu., Shchetinin A.M., Deryabin P.G., Gitel'man A.K. i dr. Taksonomiya virusa Baku (Baku virus, BAKV; Reoviridae, Orbivirus), izolirovannogo iz obligatnykh parazitov ptits - agrasovykh kleshchei (Acari: Argasidae) v Azerbaidzhane, Turkmenistane i Uzbekistane. Voprosy virusologii. 2013; 58 (6): 22-6.

10. Andreev V.L., Gromashevskii V.L., Veselovskaya O.V. Izolyatsiya virusa Baku v Zapadnoi chasti Turkmenskoi SSR. Ekologiya virusov. 1973; (1): 96-103.

11. L'vov D.K., Gromashevskii V.L., Sidorova G.A., Tuchkin Yu.M. Vydelenie novogo arbovirusa Baku gruppy Kemerovo iz argasovykh kleshchei Ornithodoros capensis v Azerbaidzhane. Voprosy virusologii. 1971; (4): 434-7.

12. Sidorova G.A., Zhmaeva Z.M., Gromashevskii V.L. Izolyatsiya virusa Baku ot kleshchei Ornithodoros coniceps, sobrannykh v gnezdov'yakh golubei v Tashkentskoi oblasti. Ekologiya virusov. 1974; (2): 102-5.

13. Mertens P.P., Brown F., Sangar D.V. Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology. 1984; 135 (1): 207-17.

14. French T.J., Inumaru S., Roy P. Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells. J. Virol. 1989; 63 (8): 3270-8.

15. Roy P., Marshall J.J., French T.J. Structure of the bluetongue virus genome and its encoded proteins. Curr. Top. Microbiol. Immunol. 1990; 162: 43-87.

16. Belaganahalli M.N., Maan S., Maan N.S., Nomikou K., Pritchard I., Lunt R. et al. Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus. PLoS One. 2012; 7 (3): e31911.

17. Belaganahalli M.N., Maan S., Maan N.S., Nomikou K., Guimera M., Brownlie J. et al. Full genome sequencing of Corriparta virus, identifies California mosquito pool virus as a member of the Corriparta virus species. PLoS One. 2013; 8 (8): e70779.

18. Dilcher M., Hasib L., Lechner M., Wieseke N., Middendorf M., Marz M. et al. Genetic characterization of Tribec virus and Kemerovo virus, two tick-transmitted human-pathogenic Orbiviruses. Virology. 2012; 423 (1): 68-76.

19. Belaganahalli M.N., Maan S., Maan N.S., Tesh R., Attoui H., Mertens P.P. Umatilla virus genome sequencing and phylogenetic analysis: identification of stretch lagoon orbivirus as a new member of the Umatilla virus species. PLoS One. 2011; 6 (8): e23605.

20. Belhouchet M., Mohd Jaafar F., Tesh R., Grimes J., Maan S., Mertens P.P. et al. Complete sequence of Great Island virus and comparison with the T2 and outer-capsid proteins of Kemerovo, Lipovnik and Tribec viruses (genus Orbivirus, family Reoviridae). J. Gen. Virol. 2010; 91 (Pt. 12): 2985-93.

21. Briese T., Calisher C.H., Higgs S. Viruses of the family Bunyaviridae: are all available isolates reassortants? Virology. 2013; 446 (1-2): 207-16.

22. Wenske E.A., Chanock S.J., Krata L., Fields B.N. Genetic reassortment of mammalian reoviruses in mice. J. Virol. 1985; 56 (2): 613-6.

23. Carpi G., Holmes E.C., Kitchen A. The evolutionary dynamics of bluetongue virus. J. Mol. Evol. 2010; 70 (6): 583-92.

24. Coetzee P., Van Vuuren M., Stokstad M., Myrmel M., Venter E.H. Bluetongue virus genetic and phenotypic diversity: towards identifying the molecular determinants that influence virulence and transmission potential. Vet. Microbiol. 2012; 161 (1-2): 1-12.

25. Brown S.E., Morrison H.G., Knudson D.L. Genetic relatedness of the Kemerovo serogroup viruses: III. RNA-RNA blot hybridization and gene reassortment in vitro of the Chenuda serocomplex. Acta Virol. 1989; 33 (3): 221-34.

26. Brown S.E., Morrison H.G., Knudson D.L. Genetic relatedness of the kemerovo serogroup viruses: II. RNA-RNA blot hybridization and gene reassortment in vitro of the Great Island serocomplex. Acta Virol. 1989; 33 (3): 206-20.

27. Belaganahalli M.N., Maan S., Maan N.S., Brownlie J., Tesh R., Attoui H. et al. Genetic characterization of the tick-borne orbiviruses. Viruses. 2015; 7 (5): 2185-209.

28. Belhouchet M., Mohd Jaafar F., Firth A.E., Grimes J.M., Mertens P.P., Attoui H. Detection of a fourth orbivirus non-structural protein. PLoS One. 2011; 6 (10): e25697.

29. Balasuriya U.B., Dobbe J.C., Heidner H.W., Smalley V.L., Navarrette A., Snijder E.J. et al. Characterization of the neutralization determinants of equine arteritis virus using recombinant chimeric viruses and site-specific mutagenesis of an infectious cDNA clone. Virology. 2004; 321 (2): 235-46.

30. White J.R., Boyd V., Brangwyn J.K., Duch C.J., Pritchard L.I., Melville L.F. et al. Characterisation and monitoring of neutralisation-resistant VP2 phenotypes in BTV-1 isolates from northern Australia collected over a twenty-year period. Vet. Ital. 2004; 40 (4): 508-12.

31. Roy P., Adachi A., Urakawa T., Booth T.F., Thomas C.P. Identification of bluetongue virus VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells. J. Virol. 1990; 64 (1): 1-8.

32. Matsuo E., Roy P. Bluetongue virus VP6 acts early in the replication cycle and can form the basis of chimeric virus formation. J. Virol. 2009; 83 (17): 8842-8.

33. Stauber N., Martinez-Costas J., Sutton G., Monastyrskaya K., Roy P. Bluetongue virus VP6 protein binds ATP and exhibits an RNAdependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates. J. Virol. 1997; 71 (10): 7220-6.