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Вопросы вирусологии. 2018; 63: 58-61

ВИРУС ЛЛОВИ - НОВЫЙ ФИЛОВИРУС, ЭНДЕМИЧНЫЙ ДЛЯ ЕВРОПЫ

Сизикова Т. Е., Лебедев В. Н., Карулина Н. В., Борисевич С. В.

https://doi.org/10.18821/0507-4088-2018-63-2-58-61

Аннотация

В обзоре рассмотрены сведения о выявлении нового филовируса, вируса Ллови (семейство Filoviridae, род Cuevavirus) на территории Европы. Изучены молекулярно-биологические свойства фрагментов генома вируса Ллови, выделенных от погибших летучих мышей Miniopterus schreibersii. Поскольку инфекционный вирус Ллови до настоящего времени не выделен, его способность инфицировать различные клетки и потенциальная опасность как агента инфекционного заболевания человека остаётся неясной. Для изучения характеристик различных элементов вируса используют рекомбинантные векторы (вирус везикулярного стоматита и плазмиды), экспрессирующие структурные белки вируса Ллови. Исследованы вопросы взаимодействия структурных белков вируса Ллови, экспрессированных с использованием рекомбинантных векторов с рецепторами клеток летучих мышей и человека. Рассмотрена возможная патогенность нового возбудителя по отношению к человеку. Сделан вывод о необходимости постоянного эпидемиологического и эпизоотологического мониторинга за новой филовирусной инфекцией.
Список литературы

1. Towner J.S., Sealy T.K., Khristova M.L., Albariño C.G., Conlan S., Reeder S.A., et al. Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda. PLoS Pathog. 2008; 4(11): 1-6.

2. Barrette R.W., Metwally S.A., Rowland J.M., Xu L., Zaki S.R., Nichol S.T., et al. Discovery of Swine as a Host for the Reston ebolavirus. Science. 2009; 325(5937): 204-6.

3. Leroy E.M., Epelboin A., Mondonge V., Pourrut X., Gonzalez J.P., Muyembe-Tamfum J.J., et al. Human Ebola Outbreak Resulting from Direct Exposure to Fruit Bats in Luebo, Democratic Republic of Congo, 2007. Vector-Borne Zoonotic Dis. 2009; 9(6): 723-8.

4. Negredo A., Palacios G., Vazquez-Moron S., Gonzalez F., Dopazo H., Molero F., et al. Discovery of an Ebolavirus-like filovirus in Europe. PLoS Pathog. 2011; 7(10): 1-8.

5. Adams M.J., Lefkowitz E.J., King A.M.Q., Bamford D.H., Breitbart M., Davison A.J., et al. Ratification vote on taxonomic proposals to the international Committee on Taxonomy of Viruses. Arch. Virol. 2015, 160(7): 1837-50.

6. Львов Д.К., Щелканов М.Ю., Альховский С.В., Дерябин П.Г. Филовирусы (Filoviridae). В кн.: Львов Д.К., ред. Руководство по вирусологии. Вирусы и вирусные инфекции человека и животных. М.: МИА; 2013: 202-5

7. Harty R.X., Brown M.E., Wang G., Huibregtse J., Hayes F.P. A PPxY motif within VP40 protein of Ebola virus interacts physically and functionally with a ubiquirin ligase: implication fjr filovirus budding. Prosc. Nate Acad. Sci. USA. 2000; 97(25): 13871-6.

8. Muller E.H., Obernoster G., Raaben M., Herbert A.S., Deffieu M.S., Krishnan A., et al. Ebola virus entry requires the host-programmed recognition of an intracellular recognition of an intracellular receptor. EMBO J. 2012; 31(8): 1947-60.

9. Volchkov V.E., Feldmann H., Volchkova V.A., Klenk H.D. Processing of Ebola virus glycoprotein be polyprotein convertase furin. Proc. Nate Acad. Sci. USA. 1998; 95(10): 5762-7.

10. Volchkov V.E., Blinov V.M., Netesov S.V. The envelope glycoprotein of Ebola virus contains an immunosuppressive-like domain similar to oncogenic retroviruses. FEES Lett. 1992; 305(3): 181-4.

11. Ng M., Ndungo E., Jangra R.K., Cai Y., Postnikova E., Radoshitzky S.R., et al. Cell entry by a novel European filovirus requires host endosomal cysteine proteases and Niemann-Pick C1. Virology. 2014; 468-470: 637-46.

12. Hunt C.L., Lennenmann N.J., Maury W. Filovirus entry: a novelty in the viralfusion world. Viruses. 2012; 4(2): 258-75.

13. Hofmann-Winkler H., Kaup F., Pohlmann S. Host cell factors in filovirus entry: novel players, new insights. Viruses. 2012; 4(12): 3336-62.

14. Maruyama J., Miyamoto H., Kajihara M., Ogawa H., Maeda K., Sakoda Y., et al. Characterization of the envelope glycoprotein of a novel filovirus, Lloviu virus. J. Virol. 2014; 88(1): 99-109.

15. Marzi A., Reinheckel T., Feldmann H. Cathepsin B and L are not required for Ebola virus replication. PLOS Negl. Trop. Dis. 2012; 6(12): 1-9.

16. Feagins A.R., Basler C.F. Lloviu virus VP24 and VP35 proteins function as innate immune antagonists in human and bat cells. Virology. 2015; 485: 145-52.

Problems of Virology. 2018; 63: 58-61

LLOVIU VIRUS - A NOVEL FILOVIRUS, ENDEMIC IN EUROPE

Sizikova T. E., Lebedev V. N., Karulina N. V., Borisevich S. V.

https://doi.org/10.18821/0507-4088-2018-63-2-58-61

Abstract

The data on a recently revealed novel filovirus (Lloviu virus, family Filoviridae, genera Cuevavirus) in Europe are viewed in this issue. The molecular-biological properties of genome fragments of Lloviu virus were isolated from perished bats (Miniopterus sсhreibersii). Because infectious Lloviu virus has not been isolated yet, the capacity of virus to infect cells of different species and its potential to cause disease in humans is unclear. The recombinant vectors (vesicular stomatitis virus and plasmids) expressing structural proteins of Lloviu virus were used to study different elements of the virus. The question of interaction of structural proteins of Lloviu virus expressed by recombinant vectors with receptors of bat and human cells is considered. The possibility of pathogenicity of the novel agent for humans is considered. The conclusion is made about the necessity of continuous epidemical and epizootical monitoring of the new filovirus infection.
References

1. Towner J.S., Sealy T.K., Khristova M.L., Albariño C.G., Conlan S., Reeder S.A., et al. Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda. PLoS Pathog. 2008; 4(11): 1-6.

2. Barrette R.W., Metwally S.A., Rowland J.M., Xu L., Zaki S.R., Nichol S.T., et al. Discovery of Swine as a Host for the Reston ebolavirus. Science. 2009; 325(5937): 204-6.

3. Leroy E.M., Epelboin A., Mondonge V., Pourrut X., Gonzalez J.P., Muyembe-Tamfum J.J., et al. Human Ebola Outbreak Resulting from Direct Exposure to Fruit Bats in Luebo, Democratic Republic of Congo, 2007. Vector-Borne Zoonotic Dis. 2009; 9(6): 723-8.

4. Negredo A., Palacios G., Vazquez-Moron S., Gonzalez F., Dopazo H., Molero F., et al. Discovery of an Ebolavirus-like filovirus in Europe. PLoS Pathog. 2011; 7(10): 1-8.

5. Adams M.J., Lefkowitz E.J., King A.M.Q., Bamford D.H., Breitbart M., Davison A.J., et al. Ratification vote on taxonomic proposals to the international Committee on Taxonomy of Viruses. Arch. Virol. 2015, 160(7): 1837-50.

6. L'vov D.K., Shchelkanov M.Yu., Al'khovskii S.V., Deryabin P.G. Filovirusy (Filoviridae). V kn.: L'vov D.K., red. Rukovodstvo po virusologii. Virusy i virusnye infektsii cheloveka i zhivotnykh. M.: MIA; 2013: 202-5

7. Harty R.X., Brown M.E., Wang G., Huibregtse J., Hayes F.P. A PPxY motif within VP40 protein of Ebola virus interacts physically and functionally with a ubiquirin ligase: implication fjr filovirus budding. Prosc. Nate Acad. Sci. USA. 2000; 97(25): 13871-6.

8. Muller E.H., Obernoster G., Raaben M., Herbert A.S., Deffieu M.S., Krishnan A., et al. Ebola virus entry requires the host-programmed recognition of an intracellular recognition of an intracellular receptor. EMBO J. 2012; 31(8): 1947-60.

9. Volchkov V.E., Feldmann H., Volchkova V.A., Klenk H.D. Processing of Ebola virus glycoprotein be polyprotein convertase furin. Proc. Nate Acad. Sci. USA. 1998; 95(10): 5762-7.

10. Volchkov V.E., Blinov V.M., Netesov S.V. The envelope glycoprotein of Ebola virus contains an immunosuppressive-like domain similar to oncogenic retroviruses. FEES Lett. 1992; 305(3): 181-4.

11. Ng M., Ndungo E., Jangra R.K., Cai Y., Postnikova E., Radoshitzky S.R., et al. Cell entry by a novel European filovirus requires host endosomal cysteine proteases and Niemann-Pick C1. Virology. 2014; 468-470: 637-46.

12. Hunt C.L., Lennenmann N.J., Maury W. Filovirus entry: a novelty in the viralfusion world. Viruses. 2012; 4(2): 258-75.

13. Hofmann-Winkler H., Kaup F., Pohlmann S. Host cell factors in filovirus entry: novel players, new insights. Viruses. 2012; 4(12): 3336-62.

14. Maruyama J., Miyamoto H., Kajihara M., Ogawa H., Maeda K., Sakoda Y., et al. Characterization of the envelope glycoprotein of a novel filovirus, Lloviu virus. J. Virol. 2014; 88(1): 99-109.

15. Marzi A., Reinheckel T., Feldmann H. Cathepsin B and L are not required for Ebola virus replication. PLOS Negl. Trop. Dis. 2012; 6(12): 1-9.

16. Feagins A.R., Basler C.F. Lloviu virus VP24 and VP35 proteins function as innate immune antagonists in human and bat cells. Virology. 2015; 485: 145-52.